BACTELABm1

defined as "The discipline addressing the safe handling and containment of infectious microorganisms and hazardous biological materials

defined in levels that increase in complexity as the risk associated with the work in the microbiological laboratory increases

provides immediate protection to workers in the biological laboratory from exposures to chemical and biological hazards

• Primary containment

• Secondary containment

• Biological Safety Cabinet

• HEPA Filter

Provides 3 basic types of protection:

1. 1. Personnel protection from harmful agents inside the cabinet

2. 2. Product protection to avoid contamination of work, experiment, or process

3. 3. Environmental protection from contaminants contained within the cabinet

• Primary containment

• Secondary containment

• Biological Safety Cabinet

• HEPA Filter

• - particles at 0.3 microns are captured with an efficiency of 99.97%

• - Particles larger than 0.3 microns and particles smaller than 0.3 microns are captured with a greater efficiency than 99.97%

• Primary containment

• Secondary containment

• Biological Safety Cabinet

• HEPA Filter

• - intended to protect the laboratory worker, the community, and the environment from unintended contamination with a biological hazard

• Primary containment

• Secondary containment

• Biological Safety Cabinet

• HEPA Filter

HEPA filters use 3 mechanism to trap particles (Which 3 are they):

• Interception

• Impaction

• Diffusion

• Dissociation

• - provides personnel and environmental protection bu no protection for research materials

• - the air exhausted from the BSC is HEPA filtered but unfiltered room in drawn into the work surface

• Class I Biological Safety CAbinets

• Class II Biological Safety Cabinets

• Class III Biological Safety Cabinets

• Class IV Biological Safety Cabinets

• - laminar flow is utilized along with HEPA filtration to allow for consistent removal or airborne contaminants

• - Airflow is drawn into the front grille of the BSC and then this air is HEPA filtered prior to being recirculated back into the cabinet providing a down flow over the work area of clean air

• Class I Biological Safety CAbinets

• Class II Biological Safety Cabinets

• Class III Biological Safety Cabinets

• Class IV Biological Safety Cabinets

• - provides highest level of personnel protection and is used for Rick Group 4 agents

• Class I Biological Safety CAbinets

• Class II Biological Safety Cabinets

• Class III Biological Safety Cabinets

• Class IV Biological Safety Cabinets