Week 10

Which situation would increase transcription in eukaryotes most?

• SWI-SNF protein complex absent, no nudging, no acetylation
• SWI-SNF protein complex present, nudging present, no acetylation
• SWI-SNF protein complex absent, no nudging, acetylation present
• SWI-SNF protein complex present, nudging, and acetylation present

DNA methylation often occurs at the 5’ cytosine in the CG dinucleotide and is associated with inactive genes -

• True
• False

What is not a characteristic of proteasome?

• Uses ATP
• Binds multi-ubiquitinated proteins
• Produce 3-25aa long peptides.
• Has ubiquitin hydrolase

What is true regarding proteins which are directed to the ER?

• There is a nuclear localisation sequence (NLS) attached to the protein.
• SRPs bind to the signal peptide of the protein.
• Disulphide bonds can form between 2 alanine side chains
• Poly-ubiquitination occurs post-translationally

What would you expect around an active gene sequence?

• Activation of HAT
• Upregulated protein inhibitors of HAT
• Histone spreading proteins
• Activators of HDAC

What may not result from a heavily mutated Methyl cytosine binding protein?

• More free HDAC in the nucleus
• Less methylation of Cytosines in CpG
• No nucleosome aggregation
• More transcription