HEMA2LECP2F

If the DNA nucleotide sequence is 59-ATTAGC-39, then the mRNA sequence transcribed from this template is:

• 59-GCUAAU-39

• 59-AUUAGC-39

• 59-TAATCG-39

• 59-UAAUCG-39

Cells with damaged DNA and mutated or nonfunctioning cell cycle regulatory proteins:

• Are arrested in G1 and the DNA is repaired

• Continue to divide, which leads to tumor progression

• Divide normally, producing identical daughter cells

• Go through apoptosis

To start DNA replication, DNA polymerase requires an available 39 hydroxyl group found on the:

• Leading strand

• mRNA

• Parent strand

• Primer

Ligase joins Okazaki fragments of the:

• 59 to 39 template strand

• Lagging strand

• Leading strand

• Primer fragments

A 40-year-old patient enters the hospital with a rare form of cancer caused by faulty cell division regulation. This cancer localized in the patient’s spleen. An ambitious laboratory developed a molecular test to verify the type of cancer present. This molecular test would require patient specimens taken from which two tissues?

• Abnormal growths found on the skin and in the bone marrow

• Normal splenic tissue and cancerous tissue

• Cancerous tissue in spleen and bone marrow

• Peripheral blood and cancerous tissue in the spleen

One main difference between PCR and reverse transcriptase PCR is that:

• PCR requires primer

• PCR uses reverse transcriptase to elongate the primers

• Reverse transcriptase PCR uses cDNA as a template

• Reverse transcriptase PCR requires liase to amplify the target DNA

Which one of the following statements about gel electrophoresis is FALSE?

• The gel is oriented in the chamber with the wells at the positive terminal

• A buffer solution is required to maintain the electrical current

• The matrix of a polyacrylamide gel is tighter than that of an agarose gel

• The larger DNA fragments will be closest to the wells of the gel

In which of the following applications would it be most appropriate to use NGS technology?

• Testing for a point mutation in the FV gene

• Testing for the BCR-ABL1 translocation in CML

• Sequencing a B cell lymphoma genome

• Determining the karyotype in AML

One major difference between endpoint PCR and real time is that:

• End-point PCR requires thermostavle DNA polymerase, deoxynucleotides, and primers

• End-point PCR requires a separate step to detect the amplicons formed in the reaction

• Real-time PCR uses capillary gel electrophoresis to detect amplicons during PCR cycling

• Real-time PCR detects and quantifies amplicons using cleavage based signal amplification

Which of the following statements about minimal residual disease is TRUE?

• Clinical remission of hematologic cancers is determined by molecular techniques such as PCR and flow cytometry.

• Real-time quantitative PCR-determined copy number of BCR-ABL1 transcripts will always be lower in molecular remission than in clinical remission.

• Qualitative PCR that uses a known copy number of a target sequence is of use in determining minimal residual disease levels

• Minimal residual disease assessment can aid physicians in making treatment decisions but does not yet offer insights into prognosis.