HISTOPATH: EMBEDDING

Is the process by which impregnated tissue is placed into a precisely arranged position in a mold containing a medium which is then allowed to solidify

• Embedding
• Casting
• Blocking

The melting point of the wax

• 5-10℃ above
• 5-10℃ below

Cooled rapidly in a refrigerator at

• -5℃
• Immersed in cold water

Orientation: process by which a tissue is arranged in precise positions in the mold during embedding, on the microtome before cutting, and on the slide before staining

• TRUE

• FALSE

Consists of 2 L-shaped strips of heavy brass or metal arranged on a flat metal plate ▪ can be moved to adjust the size of the mold to the size of the specimen ▪ recommended for routine use

• Leuckhart’s Embedding Mold
• Compound Embedding Unit
• Plastic Embedding Rings and Base Mold
• Disposable Embedding Molds

Made up of a series of interlocking plates resting on a flat metal base ▪ has the advantage of embedding more specimens at a time

• Leuckhart’s Embedding Mold
• Compound Embedding Unit
• Plastic Embedding Rings and Base Mold
• Disposable Embedding Molds

Consists of a special stainless steel base mold fitted with a plastic embedding ring

• Leuckhart’s Embedding Mold
• Compound Embedding Unit
• Plastic Embedding Rings and Base Mold
• Disposable Embedding Molds

A. Peel-Away disposable thin plastic embedding molds - B. Plastic Ice Trays: recommended for busy routine laboratories - C. Paper Boats

• Leuckhart’s Embedding Mold
• Compound Embedding Unit
• Plastic Embedding Rings and Base Mold
• Disposable Embedding Molds

Equipped with a warm plate and a cold plate at -5℃

• Tissue Tek
• Tissue Fek

Tissue Tek Advantages

• ease of use
• less paraffin wax needed
• faster embedding
• firmly attached tissue and holder and permanent identification