biotech q3

What do you call an organism that contains genes from two different organisms?

• Altered genome organism

• Mutant organism

• Modified organism

• Transgenic organism

Which of the following is the most challenging issue facing genome sequencing or organism modification?

• the availability and stability of DNA

• the ethics of using information from genomes at the individual level

• the inability to develop fast and accurate sequencing or modifying techniques.

• all of the above

What is the purpose of Polymerase Chain Reaction (PCR) in genetic engineering?

• To replicate DNA in a short amount of time

• To cut DNA to get a specific trait

• To visualize DNA fragments through gel electrophoresis

• To create covalent bonds between nucleotide chains

You are designing a cloning experiment and need to choose an enzyme to precisely cut DNA at specific locations. What enzyme would you select?

• DNA Ligase

• Polymerase

• Reverse Transcriptase

• Restriction Enzymes

Which organism is often used as a host in DNA technology experiments for producing human proteins?

• Saccharomyces cerevisiae

• Drosophila melanogaster

• Arabidopsis thaliana

• Escherichia coli (E. coli)

Which type of polymerase is responsible for replicating DNA from DNA in genetic engineering?

• RNA-dependent DNA polymerase

• Reverse transcriptase

• DNA-dependent RNA polymerase

• DNA-dependent DNA polymerase

What is the main role of vectors in genetic engineering?

• To amplify DNA fragments

• To cut DNA at specific locations

• To synthesize RNA from DNA templates

• To transfer genes from a host organism to a recipient organism

In a genetic engineering experiment, you need to select small self-replicating DNA molecules to transport genes between microorganisms. What are these small DNA molecules called?

• Ribosomes

• Plasmids

• Codons

• RNA

After conducting DNA gel electrophoresis, a researcher notices that the bands in the DNA size standard are not clearly visible under UV light. What could be the potential issue?

• The ethidium bromide staining solution was not applied properly.

• The DNA size standard was not properly mixed before loading.

• The gel was not properly solidified.

• The DNA size standard was not properly stored prior to the experiment.

During DNA gel electrophoresis, a researcher realizes that the DNA samples are not moving through the gel towards the expected direction. What could be the reason for this unexpected result?

• The agarose gel was not properly melted.

• The buffer used was too diluted.

• The comb was not properly inserted in the gel.

• The DNA samples were too concentrated.