Cell culture lab

What does BSO stand for?

• Basic Safety Officer

• Basic Science Officer

• Bio Safety Officer

• Bio Science Officer

Who is our BSO?

• Stephanie

• Ida

• Richard

• Henk

Before we send the cells for mycoplasma testing, what do we do?

(Multiple answers are correct)

• We make cryo-vials (if not present yet)

• We culture the cells in the mycoplasma incubator

• We culture 1 week without P/S or other antibiotics

• Make a pellet for mycoplasma test

After treating the cells for mycoplasma, can you directly continue with your experiments?

• No, I have to culture my cells one week without P/S and than re-test for mycoplasma

• Yes, the mycoplasma treatment is effective enough

• No, I send my cells directly after the treatment for testing

Elimination of the mycoplasma can be carried out using:

• BM cyclin

• Chlorine

• Plasmocin

• Amphotericin B

Testing for mycoplasma contamination should be done, when:

• Cells are in culture for >3 months

• Suspected/contaminated cells with mycoplasma

• Cells introduced from another lab

• Cells from the LN/-80 with non-verifiable test results

• All the answers are correct

Who is the main lab responsible in the cell culture lab?

• Lab Mom

• Pei Pei

• Alessandro

• Elisa

When do you put the lab coat on in the lab?

• ALWAYS! When entering the lab

• When you want to look stylish

• Only when you do experiments

How many gloves do you use when working with cells?

• One glove on each hand

• Double gloves on each hand, better be safe than sorry.

• One glove on your non-dominant hand

• No gloves at all

What colour is the label on our ML-1 lab coats?

• White

• Red

• Yellow

• There is no label