Ex 10 TPAG

Purpose of KI in Biuret Reagent

• Provides alkaline environment

• Major reactant

• Comples are cupric ions to prevent their pptation in the alk soln

• Antioxidant/stabilizers

Why is serum the preferred sample for Total CHON by Kingsley?

• Presence of fibrinogen in plasma

• Presence of fibrinogen in serum

• Plasma levels for TP are 2-4g/L higher than serum levels

• RBC’s will release intracellular CHONs to plasma

Author of the old method

• Kingsley modified by Weischelbaum

• Schales and Schales

• Karmen modified by Henry

• Jaffe method

Modification done by Kingsley

• Using high concentration of NaOH

• Use of ethyl ether as a means of separating the precipitated albumin

• Addition of Na K Tartrate and KI

• 3:1 ratio of tartrate to copper sulfate in low alkali biuret reagents

TRUE/FALSE: the more peptide bond, the more instense the color, the higher the concentration

• TRUE

• FALSE

Standard used in Ex 10B

• 5g/dL bovine serum albumin

• 10g/dL porcine serum albumin

• 5mg/dL human serum albumin

• 10mg/dL ovalbumin

What is the method used in exercise 10B

The dye used in exercise 10B

• Bromophenol blue

• Bromcresol purple

• Bromcresol green

• Buffer yellow

Wavelength used in 10A and 10B

• 10A - 546nm ; 10B - 555nm

• 10A - 555nm ; 10B 546nm

• 10A - 340nm ; 10B - 450nm

• 10A - 650nm ; 10B - 340nm

Convert 3.8g/dL albumin to SI unit

• 550.62 umol/L

• 567.72 mmol/L

• 550.62 mmol/L

• 567.72 umol/L