Mol Bio Quiz5

 What is the primary purpose of electrophoresis?

• To visualize DNA or RNA fragments under a microscope

• To amplify DNA or RNA fragments for cloning

• To sequence DNA or RNA fragments

• To separate DNA or RNA fragments based on size

Given the target sequence of the gene and detection sequence (highlighted in red ) below, design a linear probe to be used in a real-time PCR assay:

5'-AATGTGCATGC......TAAGCGTAGTCGGTCAG......TTACGACTTGAGCACTGA-3'

You are stuck in a forest camp and a viral infection has broken out. You have access to toe-warmers which can reach a maximum temperature of 38deg celsius and an enzyme kit which was brought by organizers for activities in the biology sessions. Which NAAT could you design to test for viral infection in the camp group?

• HDA

• LAMP

• RPA

• PCR

Which of the following are names of nucleic acid sequencing methods?

• Sanger sequencing

• Illumina sequencing

• Single nucleotide building (SNB)

• All of the above

How is the migration rate of DNA fragments influenced by agarose gel concentration?

• Higher concentration of agarose results in faster migration rate of DNA fragments

• Agarose concentration does not affect the migration rate of DNA fragments

• Lower concentration of agarose results in faster migration rate of DNA fragments

• Agarose concentration influences the migration rate of DNA fragments, but the relationship is complex

Which sequence ensures that that CRISPR-Cas system doesn't run on self-destruction mode?

• crRNA

• trRNA

• PAM sequence

• sgRNA