Electrophoresis pre-lab check

This laboratory procedure is known as

• CLONING

• gel electrophoresis

• chromatography

• use of a dichotomous key

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Which chemicals are used to cut DNA into fragments for a gel electrophoresis procedure?

• enzymes

• molecular bases

• hormones

• ATP molecules

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

• micropipette

• transfer pipette

• pipette pump

• serological pipette

Always change out the micropipette tip when changing reagents.

• true

• false

How are buffers used in Electrophoresis?

• Conduct electricity

• Remove salts

• Add extension energy

• Dissolve excess DNA