Quiz 6 (Lec. 10 & 11)

Production in cell culture flasks halt when:

• Cells require more oxygen that can be supplied

• Can no longer adequately meet the demand of

  the culture

• Nutrients have been depleted

• All of the above

Bioreactors are able to control what growth factors on a global basis?

• Agitation

• Temperature

• CO₂

• A, B

• A, B, C

Which of the following is an advantage that reactors have over flasks?

• Consistency of content inside shaker/incubators

• Pressure, amino acids, and salinity are controlled on a global basis

• No indications on the health of the cells inside the flask

• A, C

• A, B, C

What type of bioreactor contains a water recirculation loop and electric heater

to heat the reactor?

• Water-Jacketed Vessel

• Single Wall Vessel

• Plain Jacket Vessel

• Water-cooled Jacketed Glass Vessel

Tags

NGSS.HS-PS3-4

Vortexing impellers have __ blades with _____ edges and creates vortexes around the ____. (order matters)

• 3, smooth, center

• 3, sharp, center

• 2, smooth, blades

• 2, sharp, blades

What is the gas used to decrease the level of dissolved oxygen in media?

• Oxygen/Air

• Ammonia

• Nitrogen

• Carbon dioxide

Which of the following aeration components creates small bubbles that are less likely to carry cellular material?

• L-Sparge

• Sintered Sparge

• Rotameter

• Thermal Mass Flow Controller (TMFC)

What is the main function of exhaust condensers?

• Strips any entrained liquids from the exiting process stream

• Filters debris from the exiting gas pathway

• Cools the exiting gas pathway

• Adds water vapor to the exiting gas

Which of the following statements regarding dual-filter exhausts is true?

• A condenser is used in addition to the dual-filter tube

• Length of the dual-filter tubing is used

  to condense out entrained liquids

• A second filter is introduced if

  the first filter gets wet

• None of the above

Which of the following affects dissolved oxygen levels?

• pH

• Total volume of vessel

• Media composition

• None of the above

Tags

NGSS.HS-ESS2-5

NGSS.HS-LS2-5