FRET QUIZ

FRET include radiationless energy transfer 

• True

• False

Process where energy release from relaxation of excited donor group excite the acceptor group? 

• Relaxation

• Resonance

• Excitation

Acceptor and donor group must be withing what distance? 

• 30 nm

• 70 nm

• 5 nm

• 10 nm

Which factor affects the scattering and thus leads to errors? 

• Intensity of Fluorescence

• Donor Group

• Acceptor Group

• Absorbance of fluorescence

Which of the following is not a factor in determining a good donor and acceptor?

• Lack absorption interference from other fluorophores

• Insensitive to solvent environments

• Resistant to photobleaching 

• Low quantum yield

Given the equation, what does E stands for?

• Rate of FRET

• Rate of radiative relaxation

• FRET efficiency

• Non radiative relaxation state such as internal or external conversion

Choose all that apply: FRET can be used for

• Visualize protein- protein interaction, for example, between ligands and receptors 

• Determine complex conformational changes  

• DNA-protein interactions  

What type of prism is used in the instrumentation for Single Molecule FRET Microscopy? 

• Glass

• Silica

• Zirconium 

• Diamond 

FRET cannot be used in vivo due to its invasive nature 

True 

• True

• False

Slight changes in distance will significantly affect FRET signals 

• True

• False