DNA Fingerprinting and HGP

Which of the following techniques is not used in the process of DNA fingerprinting?

• Digestion of DNA using restriction endonucleases

• Southern blotting

• Autoradiography

• Separation of DNA fragments by centrifugation.

Select the incorrect statement in regards to the polymorphism in DNA sequences

• It become very useful identification tool in forensic applications

• It Is non-inheritable from parents to children

• DNA from every tissue from an individual shows the same degree of polymorphism.

• It becomes the basis for DNA fingerprinting.

Read the following statements and select the correct statement.

Statement I: The degree of DNA polymorphism in every tissue of an individual is identical.

Statement II: A single nucleotide variation is called DNA polymorphism when its occurrence frequency is more than 0.01 in a population.

• Only Statement I is correct

• Only statement II is correct.

• Both the statements are incorrect

• Both the statements are correct.

Commonly used vectors for human genome sequencing are:

• T - DNA

• BAC and YAC

• Expression Vectors

• T/A Cloning Vectors

An analysis of chromosomal DNA using the Southern hybridization technique generally does not use:

• Electrophoresis

• Blotting

• Autoradiography

• PCR

Arrange the various steps of DNA fingerprinting technique in the correct order.

(i) Separation of DNA fragments by electrophoresis.

(ii) Digestion of DNA by restriction endonucleases.

(iii) Hybridisation using labelled VNTR probe.

(iv) Isolation of DNA

(v) Detection of hybridised DNA fragments by auto radiography.

(vi) Transferring the separated DNA fragments to nitrocellulose membrane.

• (iv) → (ii) → (i) → (vi) → (iii) → (v)

• (iv) → (i) → (ii) → (iii) → (vi) → (v)

• (ii) → (i) → (iv) → (vi) → (iii) → (v)

• (iii) → (v) → (iv) → (ii) → (i) → (vi)

SNPs is an abbreviated form of

• Single nucleoside polymorphism

• Simple nucleotide polymorphism

• Simple nucleoside polymorphism

• Single nucleotide polymorphism

The technique of DNA fingerprinting was developed by

• Nicolson

• Robert May

• Alec Jeffreys

• Fredrick Sanger

The average size range of VNTR is

• 1-20 kbp

• 0.1-20 bp

• 20-200 bp

• 0.1-20 kbp

EST is the abbreviated form of

• Eukaryotic Splicing Technique

• Expressed Short tandems

• Expressed Sequence Tags

• Elongated Sequence Terminator