Chromatography

Among the 3 specimens for paper chromatography, which is subjected to UV light?

• Ink mixture

• Plant extract

• Fingerprint

During chromatography, should the specimen touch the solvent?

• Yes

• No

What do you call the end result of chromatography?

In paper chromatography, what is responsible for the movement of the mobile phase upward into the stationary phase?

• Capillary action

• Gravity

• Adhesion

• Absorption

Basis of separation for Paper Chromatography

• Partition

• Liquid-Liquid

• Polarity

• Particle size

What is the principle for Thin Layer Chromatography?

• Partition

• Adsorption

• Liquid-Liquid

• Polarity

What is the mobile phase for TLC

• Benzene

• Water

• Acetone and petroleum ether

• N-butanol and NH4OH

The specimen used in TLC

• Ink mixture

• Stahl’s dye

• Amino acid mixture

• Plant pigments

Identify the different layers, in chronological order, formed in TLC chromatogram (from top to bottom)

• Indophenol blue

  Sudan red O

  Buffer yellow

  Solvent front

• Solvent front

  Sudan Red O

  Buffer Yellow

  Indophenol Blue

• Solvent front

  Buffer yellow

  Sudan red O

  Indophenol Blue

• Sudan Red O

  Indophenol Blue

  Buffer Yellow

  Solvent front

True or False: in TLC, identification is done by solving or determining the ratio of fronts or retention factor values of the sample

• True

• False