CRISPR Review

What does CRISPR allow scientists to do?

• Delete Section of DNA Only

• Add New Sections of DNA Only

• Deleting, Adding, or Changing Sections of DNA

• Creating Life From Nothing

Tags

NGSS.HS-LS3-2

What is the role of Cas9?

• Glue sections of DNA together.

• Target mutated genes.

• Change the sequence or order of DNA bases.

• Cut DNA at specific locations.

Why do bacteria have CRISPR? Check all that apply.

• To be able to remember which viruses they have been infected with

• To be able to edit virus DNA

• To cut apart virus DNA thereby stopping the virus infection

• To be able to edit their own DNA

Tags

NGSS.HS-LS4-4

A PAM is

• a 15-20 bp sequence snipped from a viral genome that serves as a tracer DNA to locate viruses later

• a 3 bp sequence that adjacent to viral DNA in a CRISPR array

• a girl's name popular in the 1960s

• where the DNA is cut

CRISPR-Cas9 was discovered in which of the following organisms?

• protists

• fungi

• bacteria

• viruses

After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following types of repair mechanisms creates a mutation and inactivates the gene.

• Homology Directed Repair

• Non-Homologous End Joining

Tags

NGSS.HS-LS3-2

After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves "editing" of the gene with insertion of a corrected sequence?

• Homology Directed Repair

• Non-Homologous End Joining

What is the function of guide RNA in CRISPR-Cas9 system?

• Guide RNA helps in the replication of DNA.

• Guide RNA is responsible for repairing damaged DNA.

• Guide RNA guides the Cas9 enzyme to the target DNA sequence.

• Guide RNA acts as a template for protein synthesis.

Which enzyme is responsible for cutting the target DNA in CRISPR-Cas9 system?

• Cas9

• RNA polymerase

• DNA polymerase

• CRISPR

What is the role of PAM (Protospacer Adjacent Motif) in CRISPR-Cas9 system?

• PAM is responsible for cutting the DNA in the CRISPR-Cas9 system.

• PAM is a type of RNA molecule that guides the Cas9 enzyme to the target DNA sequence.

• PAM is a protein that binds to the CRISPR RNA in the CRISPR-Cas9 system.

• PAM is a recognition site for the Cas9 enzyme in the CRISPR-Cas9 system.