12 Biology: Biotech I

The cell membrane and nuclear membrane need to be removed before DNA can be accessed. This is achieved through a process called

• lysis

• cleaving

• dissolving

• enzymic lipase

Specific sequences of DNA are cut by molecular scissors called

• lysosomes

• restriction enzymes

• expansion enzymes

• PCR enzymes

The purpose of gene probes are to

• locate short tandem repeats

• bind with target DNA for easy identification

• separate sections of DNA by size

• assist with hybridisation of DNA

PCR stands for

• polymerase case restriction

• polymerase chain reaction

• polymerase cytosol restriction

• performance chain reaction

the removal of DNA from a cell is called DNA

• retension

• extraction

• processing

• withdrawal

In stage 1 of PCR amplification, the DNA is heated up to _____ degrees C

• 50

• 80

• 95

• 100

At what temperature DNA primers bind with DNA strands?

• 40

• 50

• 60

• 70

The process of attaching primers to DNA sections is called

• annexing

• repairing

• attaching

• annealing

The enzyme involved in PCR amplification is called

• DNA polymerase

• TAQ polymerase

• RNA polymerase

• mRNA polymerase

What two methods is DNA removed from a solution of cellular components?

• precipitation via ethanol

• use of magnets as DNA is positively charged

• use of magnets as DNA is negatively charged

• chemical induced precipitation