molecular techniques 2

which of the follwing regarding DNA cloning is not true

• a plamid vector is used

• different restriction enzymes used for DNA and vector

• used to produce multiple copies of gene of intrest

• all of the above

In Southern blotting, the transfer of DNA fragments to the blot is done by:

• Wicking action

• Cappillarity

• electric current

• A&B

In Sanger sequencing method, chain termination occurs due to:

• Denaturation using heat

• Denaturation using alkaline medium

• Consumption of deoxy-bases

• Absence of 3-OH group in dideoxy bases

The genetic matrial extracted for assay by DNA microarray is:

• DNA

• cDNA

• mRNA

• tRNA

The genetic material extracted for DNA microarray is:

• DNA

• cDNA

• mRNA

• tRNA