Immunofluorescence

Monoclonal Antibodies...

• come from different B-cell lineage

• come from the same B-cell lineage

• bind the same epitope

• can bind different antigens

Which part of an antibody in general varies less?

• The stem (Fc= constant region)

• the 'arms'

• Fab (antibody binding domain)

• none

Before we proceed to blocking of unspecific bonds we have to ____ the cells.

This scheme represents:

• direct Immunofluorescence

• double-label Immunofluorescence

• indirect immunofluorescence

• none of the fiven answers

Indirect immunofluorescence: Which step should be in place of the question mark?

• Incubation with Tertiary Antibody (that contains a fluorophore)

• Rinse with washing medium (removal of unspecific bonds)

• Add an antifade mounting medium (protect the dye from fading during imaging)

• Add a fixation medium to inactivate enzymes that might disrupt cell morphology

• None of the given answers

Which part of the cell is shown in blue in this picture?

• cytoskeleton (microtubili)

• nucleus

• cell membrane

• mitochondira

• fat vacuole