Chapter 4: DNA Manipulation

What type of enzymes are used to cut DNA?

• Endonucleases

• Ligases

• Polymerases

• Synthases

What is the name of the enzymes that build phosphodiester bonds to join pieces of DNA together?

• Endonucleases

• Ligases

• Polymerases

• Synthases

Taq Polymerase is used to synthesize complementary strands during PCR. What temperature does it operate best at?

• 37 degrees

• 50-55 degrees

• 72 degrees

• 95 degrees

Which of the following factors would not influence how fast DNA molecules move during Gel Electrophoresis?

• Strength of the electrical charge

• Viscosity of the agarose gel

• Size of DNA fragments

• Species from which DNA is extracted

In the word CRISPR, what does that "P" stand for?

• Palindromic

• Polymerase

• Partial

• Perpendicular

Who is the father of the child?

• Man 1

• Man 2

• Man 3

• None of these

When a sample of an invading Bacteriophage DNA is "filed" into the CRISPR gene, it is known as:

• Protospacer

• guide RNA

• PAM

• Spacer

A rice plant has a gene inserted from wheat, to give it an advantageous trait. The rice would be:

• A GMO and Transgenic

• A GMO only

• A GMO and Cisgenic

• Not a GMO

The process of adding a human gene into a bacterial plasmid is known as:

• Transformation

• Recombination

• Translation

• Conjugation

A large sample of DNA is required to conduct a variety of testing in order to design treatment for a disease. The best way of obtaining a large sample is to do:

• Bacterial Transformation

• Gel Electrophoresis

• Polymerase Chain Reaction

• Genetic Engineering