Biotechnology (processes & applications)

In gel electrophoresis, separated DNA fragments can be visualized with the help of.

• Acetocarmine in UV radiation

• Ethidium bromide in infrared radiation

• Acetocarmine in bright blue light

• Ethidium bromide in UV radiation

Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?

• BOD Reactor

• Sludge digester

• Industrial oven

• Bioreactor

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with ...

• Isopropanol

• Chilled ethanol

• Methanol at room temperature

• Chilled chloroform

Which of the following is true for Golden rice?

• It is Vitamin A enriched, with a gene from daffodil

• It is pest resistant, with a gene from Bacillus thuringiensis

• It is drought tolerant, developed using Agrobacterium vector

• It has yellow grains, because of a gene introduced from a primitive variety of rice

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?

• γ phage

• Ti Plasmid

• Retrovirus

• PBR 322

The correct order of steps in Polymerase Chain Reaction (PCR) is ..

• Denaturation, Extension, Annealing

• Annealing, Extension, Denaturation

• Extension, Denaturation, Annealing

• Denaturation, Annealing, Extension

The sequence that controls the copy number of the linked DNA in the vector, is termed

• Palindromic sequence

• Recognition site

• Selectable marker

• Ori site

Choose the correct pair from the following ...

• Nucleases - Separate the two strands of DNA

• Exonucleases - Make cuts at specific positions within DNA

• Ligases - Join the two DNA molecules

• Polymerases - Break the DNA into fragments

Which of the following statements is not correct?

• The functional insulin has A and B chains linked together by hydrogen bonds.

• Genetically engineered insulin is produced in E.Coli.

• In man insulin is synthesised as a proinsulin

• The proinsulin has an extra peptide called C-peptide.

Disorder in which β-lymphocytes and T-lymphocytes are not formed is ...

• AIDS

• Cystic Fibrosis

• SCID

• Muscular Dystrophy