Microscopy Practical Revision

When you use a microscope to look at a specimen, which objective lens do you use first?

• 4x

• 10x

• 40x

• Doesn't matter

Which focus knob should you use when viewing the specimen at 40x objective lens?

• Fine focus knob only

• Coarse focus knob only

• Either one is fine

Which option shows the correct steps to prepare a wet mount?

• Put lots of water -> place a thin sample

• Put a few drops of water -> place a thick sample

• Put a few drops of water -> place a thin sample

• Put lots of water -> place a thick sample

Which is the correct way to put the coverslip?

• Drop the coverslip on top of the sample

• Start at a 45-degree angle and lower it slowly

• Start at a 90-degree angle and lower it slowly

• Press the coverslip onto the sample tightly

Which of the following are not allowed for biological drawings? (Choose more than 1)

• Clean and neat lines

• Sketching

• Colouring the nucleus

• Not crossing the label lines

What is the formula to calculate the actual length of the cell?

• no. of divisions x length per division

• no. of divisions / length per division

• no. of divisions x magnification of microscope

• measure using your ruler

How do you calculate the magnification of your drawing?

• length of your drawing x actual length of cell

• eyepiece magnification x objective lens magnification

• actual length of cell / length of your drawing

• length of your drawing / actual length of cell

What organelles do a plant cell have but not an animal cell? Choose more than 1

• Cell wall

• Cell membrane

• Cytoplasm

• Chloroplast

Which of the following are required for biological drawings? Choose more than 1

• Labels

• Title

• Indicate length of your drawing

• Small size

Where should you position your specimen so that you can see it when you switch to a higher power?

• At the left side

• At the right side

• In the centre

• At the bottom