Biology Unit 4 AOS 2

Biology Unit 4 AOS 2

11th - 12th Grade

10 Qs

quiz-placeholder

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Biology Unit 4 AOS 2

Biology Unit 4 AOS 2

Assessment

Quiz

Biology

11th - 12th Grade

Hard

Created by

Rachael Hyland

Used 38+ times

FREE Resource

10 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

Which of the following statements is false?

Endonucleases act as molecular scissors.

Ligases have specific recognition sites.

Polymerases catalyse the synthesis of polynucleotides.

Sticky-end endonucleases generate DNA fragments with overhanging nucleotides.

2.

FILL IN THE BLANK QUESTION

20 sec • 1 pt

The following DNA strand was cut by three restriction enzymes, how many fragments will be formed?


5' A T C G A T C T T A A G C T T C G A A G G A T C C A T T3'

3' T A G C T A G A A T T C G A A G C T T C C T A G G T A A5'

3.

FILL IN THE BLANK QUESTION

20 sec • 1 pt

Fill in the blank

___________ a protein that joins DNA fragments together

4.

FILL IN THE BLANK QUESTION

20 sec • 1 pt

Fill in the blank

___________ the result of a staggered cut in a DNA strand by an enzyme

5.

FILL IN THE BLANK QUESTION

20 sec • 1 pt

Fill in the blank


___________ cut fragments of DNA at known sites

6.

MULTIPLE SELECT QUESTION

20 sec • 1 pt

Select all the following that are true about Polymerase Chain Reaction (PCR)

Uses Taq polymerase

Use Taq endonuclease

Goes runs in the following order 1. Denaturation, 2. Annealing, 3. Elongation 4. Repeat

triples the amount of DNA each cycle.

Uses DNA primers

7.

MULTIPLE SELECT QUESTION

1 min • 1 pt

The following are the steps involved in setting up a gel electrophoresis experiment. Which option indicates the correct order of these steps?

1. DNA samples are loaded into wells in each lane of the agarose gel.

2. Bands can be cut from the gel and purified for use in further experiments.

3. Gel is submerged in buffer and an electric current is passed through the samples.

4. DNA is prepared using restriction enzymes or PCR.

5. DNA samples are loaded into bands of the agarose gel.

6. Bands are visualised under ultraviolet light.

7. DNA fragments migrate towards the positive electrode.

8. DNA fragments migrate towards the negative electrode.

4,1,3,8,6

4,1,3,7,6,2

5,3,7,4,6,2

1,3,8,4,6

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