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Primer in PCR is......., Primer in replication is ........

• DNA,DNA

• DNA,RNA

• RNA,RNA

• RNA,DNA

PCR amplifies

• Gene

• Sequence of DNA

• Chromosome

• Chromatid

Double stranded DNA melts to single stranded DNA in

• Annealing

• Denaturation

• Extension

Temperature of annealing depends on

• Melting temperature

• Number of G+C nucleotides

• Number of A+T nucleotides

• All of the above

Reverse primer binds in the

• Coding strand

• Non coding strand

• Non sense strand

• Template strand

Reverse primer binds in the

• Coding strand

• Non coding strand

• Non sense strand

• Template strand

DNA polymerase extends DNA at 5' end

• False

• True

PCR amplifies

• Gene

• Sequence of DNA

• Chromosome

• Chromatid