CRISPR Cas9

CRISPR-Cas9 is a type of ________ system found in bacteria.

• digestive

• endocrine

• circulatory

• immune

CRISPR-Cas9 was discovered in which of the following organisms?

• protists

• fungi

• bacteria

• viruses

Scientists have adapted it to turn it into a biotechnology tool for _______ DNA.

• building

• editing

• destroying

• synthesizing

The enzyme portion of this complex is called ________.

• CRISPR

• Replicase

• Lysozyme

• Cas9

What type of RNA is used as a GPS of sorts, to find or locate specific genes?

• mRNA

• gRNA

• tRNA

• rRNA

What is PAM?

• a 3 letter sequence for binding on the target DNA

• the main gene portion of the DNA

• the cut site for Cas9

• a 10 letter sequence for binding on the target DNA

Once the DNA and RNA are bound, Cas9 is activated to cut the DNA ____ upstream from the binding sequence.

• 1 base

• 3 bases

• 6 bases

• 10 bases

After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following types of repair mechanisms creates a mutation and inactivates the gene.

• HDR

• NHEJ

After Cas9 cuts the target DNA, cellular enzymes attempt to repair the break. Which of the following repair mechanisms involves "editing" of the gene with insertion of a corrected sequence?

• HDR

• NHEJ