Genetic Engineering

Why are plasmids suitable for use in gene technology?

Tick all that apply

• Contain marker genes e.g. antibiotic resistance

• Can multiply independently

• Contain restriction sites

• Small & Circular

The DNA of plasmids can be cut at specific sites using ......

• Ligase

• Endoprotease

• restriction (endonuclease) enzymes

• Polymerase

Why are promoters included alongside the desired gene into plasmids during the genetic modification?

Tick all that apply

• RNA polymerase can bind

• So that the gene can be switched off

• Transcription factors can bind

• To control the amount of gene expression

Why has it become rare to use antibiotic markers during genetic modification these days?

• Plasmids cannot be transferred between bacteria

• Bacteria can become resistant to antibiotics

• Plasmids can be transferred between bacteria

What process can occur naturally and lead to the transfer of plasmid DNA between bacteria?

Why is it that when the human gene for insulin is inserted into the gene for tetracycline the bacterium will not have resistance to the antibiotic anymore?

• There are not enough promoters to enable gene expression

• The insertion 'break' the gene therefore it no longer works

True or False?

When GFP gene is expressed it leads to the eventual production of green fluorescent protein (GFP).

By splicing the gene of interest (e.g. Human insulin gene) together with a GFP gene and promoter into a plasmid, the recombinant plasmid has a GFP marker gene.

The transformed bacteria can be identified because the GFP (protein) fluoresces (glows) green in UV light.

• True

• False

A group of restriction enzymes that are able to cut DNA into shorter lengths at specific points are known as?

Any structure such as a plasmid which carries DNA into a cell is known as a...?