Restriction Digestion and Gel Electrophoresis

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

What applications can gel electrophoresis be used for?

• Parental Testing
• Criminal Investigations
• Sequence Endangered Species DNA
• All of the above

Why do bacteria have restriction enzymes?

• To prevent being infected by viruses
• To help DNA get into the cell
• To be able to grow in an incubator
• They don't - viruses have restriction enzymes

Which RE's from above make blunt cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Which RE's from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI