Tools for Genetic Engineering

Genetic Engineering can be used to...

• Produce pest resistant crops

• Gain a greater understanding of diseases such as cancer

• Treat genetic disorders

• Produce chemicals such as Factor VIII

• All of the above

Tags

NGSS.HS-LS1-1

Genes found on DNA contain the instructions for making  

• pyrimidines 
  
• pyrimidines
• proteins
• purines

Tags

NGSS.HS-LS1-1

What is a vector?

• Cells that produce genetically modified DNA

• Structures that deliver genes into required cells

• Enzymes that cut DNA at specific palindrome sequences

• A machine that copies DNA

What is recombinant DNA?

• DNA from two different organisms combined

• DNA from two different ribosomes

• DNA that is separated out from a vector

A circular piece of DNA, found in bacteria, is called:

• A plasmid

• mRNA

• Ribosome

• Nucleotide

Which is used to cut DNA molecules at specific points?

• reverse transcriptase

• PCR analysis

• a restriction enzyme

• recombinant DNA

Where do restriction enzymes cut DNA?

• In the middle of a gene

• At a palindromic DNA sequence

• At a civic DNA sequence

• At random locations along a gene

Restriction enzymes cut DNA to produce _________________ ends or _______________ ends.

• Sticky, Non-sticky

• Staggered, dull

• Tapering, blunt

• Sticky, blunt

How can the gene of interest be obtained?

• By making a synthetic gene

• By cutting the gene out of the genome

• By cutting the gene out of a liposome

• By reverse transcription

Reverse transcription...

• Produces the gene of interest from its mRNA sequence

• Produces mRNA from DNA coding for the gene of interest

• Sticks pieces of DNA together

• Causes the gene of interest to be translated