Histo Exam 1

What is the most important step in histology processing?

• Fixation

• Staining

• Embedding

• Clearing

What characteristics of tissues can be seen in poorly fixed/autolyzed tissue?

• Putrefaction

• Washed out appearance

• Swelling of cytoplasm

• Crisp cellular detail

What are the functions of fixatives?

• Help with refractive index

• Firm tissue for grossing

• Enhance staining

• Stabilize tissue elements

What are the physical methods of fixation?

• Heat/Microwave

• Pressure

• Desiccation

• Freezing

A section submitted should not be thicker than a .....

A section submitted should be no larger than a _________ stamp.

The volume of a fixative should be at least _______ times greater than the tissue volume.

• 5 to 10

• 10 to 15

• 15 to 20

• 20 to 25

What is the ischemic time?

• Interval between the tissue being placed in formalin and being infiltrated with paraffin

• Interval between the interruption of blood supply and being infiltrated with paraffin

• Interval between the interruption of blood supply and being placed in fixative

What is the rate of penetration of formalin?

• 0.5 cm per hour

• 1 cm per hour

• 3 mm per hour

• 1 mm per hour

What is the minimum amount of fixation time for Her2Neu testing?

• 4 hours

• 6 hours

• 48 hours

• 72 hours