Baculovirus vectors

‘Nuclear occlusion bodies’ are found in _______________

• a) Baculoviruses

• b) Adenovirus

• c) Human somatic cells

• d) Human germ cells

Nuclear occlusion bodies are _________

• a) Nuclear particles

• b) Vitamins

• c) Proteinaceous particles

• d) Replication genes

10. Which protein is expressed at high levels in occlusion bodies of baculoviruses?

• a) Polyhedron

• b) Amylose

• c) Glucose

• d) Lactose

The polyhedrin gene of baculovirus vectors cannot be replaced by foreign DNA.

• a) True

• b) False

cMNPV vector is a type of __________ which is used for expression in insect cells.

• a) Baculoviruses

• b) Adenoviruses

• c) Caulimoviruses

• d) Papillomavirus

Baculovirus vectors cannot co-express multiple transgenes.

• a) True

• b) False

Polyhedrin gene replacement vectors are most popular due to __________

• Wide host range

• Recombinant protein production

• Gene Cloning

• Lytic cycle

Blue-white screening in Baculovirus vectors can be facilitated by ____________

• a) Ampicillin resistance gene

• b) Toluene resistance gene

• c) lacZ’ gene

• d) Rep gene

What is a Bacmid?

• a) Hybrid replicon

• b) AAV

• c) Baculovirus

• d) Phage

How can the recombinants be identified if LacZ’ is substituted by GFP?

• a) Blue white screening

• b) UV light exposure

• c) Plaque color screening

• d) Lactose breakdown