Plasmid DNA isolation

The term plasmid was given by

• Herbert Boyer

• Hamilton Smith

• Joshua Lederberg

• Bolivar

The different confirmations of plasmids that exist are

• Linear

• Open Circular

• Covalently Closed circular

• None of the above

Ethidium bromide is an

• Precipitation of DNA

• Intercalating agent

• used in isolation of DNA

• unwinding agent

Which form of plasmid migrates fastest?

• Supercoiled

• Open circular

• Linear

• Closed Circular

A high copy number plasmid with an ORI, MCS, lacZ gene and ampicillin resistance gene is

• pBR322

• pSC101

• pUC18/19

• None of the above

Alkaline lysis is done by

• sodium chloride+SDS

• Potassium acetate

• GTE mix

• TritonX

Why does the plasmid get renatured and not the genomic DNA on addition of neutralizing solution?

• The genomic DNA is smaller than plasmid

• Due to antibiotic resistance of plasmid

• Due to small size of plasmid

• None of the above

Which component in MixI provides the alkaline pH 8.0 that protects acid labile DNA

• Tris-HCl

• EDTA

• Glucose

• None of the above

In gel electrophoresis, separation of DNA is according to

• Charge

• Size

• Density

• Amount of DNA in the sample

What preciptates the DNA?

• EDTA
• NaCl
• Ethanol
• Lysis Buffer