Genetic Technology (801)

What technique is used to produce many copies of a DNA sequence from a very small quantity

• Gel Electrophoresis
• Polymerase Chain Reaction
• Gene Therapy
• DNA replication

Which of the following DOES NOT explain the significance of carrying out PCR on DNA samples when doing genetic testing for an embryo or foetus?

• so that the DNA fragments will be visible on the gel
• the gene probes used to locate the DNA fragments will have many samples to bind to
• some of the DNA may get damaged during the PCR process
• only a small quantity of DNA sample can be obtained from the foetus/embryo and so it should be increased in quantity

The enzyme used to treat DNA fragments before loading them on to the agarose gel for gel electrophoresis is …………………………

• Taq polymerase
• DNA polymerase
• Restriction endonuclease
• Restriction Exonuclease

What causes DNA fragments to get separated during gel electrophoresis?

• the positive charge of the the DNA causes them to move at different rates through the electric field of the gel medium
• the negative charge of the DNA causes them to move at different rates through the electric field of the gel medium
• the different sizes of the DNA fragments causes them to move through the gel medium
• the electric field within the gel medium causes the DNA fragments to move through it at different rates

Which DNA fragment will be closest to the positively charged pole of the gel plate?

• 1500kbp fragment
• 150kbp fragment
• 1500bp fragment
• 15kbp fragment

Which DNA fragment will be closest to the negatively charged pole of the gel plate?

• 1500kbp fragment
• 150kbp fragment
• 1500bp fragment
• 15kbp fragment

Which part of the DNA is responsible for its movement through the agarose gel

• the hydrogen bonds
• the phosphate groups
• the nitrogenous bases
• the phosphodiester bonds

The optimum temperature for Taq polymerase

• 95OC
• 72OC
• 79OC
• 50OC

What is the ideal temperature for annealing to occur

• 95OC
• 72OC
• 79OC
• 50OC

PCR occurs in cycles and each cycle comprises of ………………………..

• 3 stages
• 5 stages
• 7 stages
• 2 stages