Protein Purification and Field TripQuiz

Hydrophobic amino acids are ones which _______ water.

• are attracted to

• are repelled by

• react with

• melt in

What “feature” did the Hydrophobic Interaction Chromatography (HIC) beads have to bind to protein?

• Nickel ions

• HIS

• Methyl (-CH₃)

• Polyethylene Glycol

In HIC, what had to be done to GFP before adding the GFP to the beads?

• A HIS-tag was added

• The protein was precipitated with PEG

• The protein was flipped inside out

• The protein was extracted from the egg

In Hydrophobic Interaction Chromatography, how was the protein flipped inside out?

• Genetic engineering

• The protein was mixed with PEG and centrifuged

• A high salt solution was added

• A solution free of salt was added.

In HIC, what had to be done in order to get the GFP to fall off the beads?

• Add imidazole

• Add an even higher concentration of PEG

• Add a high salt solution

• Add a solution free of salt

In HIC, how did adding a low salt solution help with removing the GFP from the beads

• It didn't help.

• It reacted with the proteins and turned purple

• It caused the GFP to flip back to their original shape

• It added HIS-tag to the end of the protein

In "HIS-tag" protein purification, what is "HIS"?

• Histidine - an amino acid

• Histamine - a chemical produced by the body during an immune reaction

• Hydrophobic Interaction Systems

• Highly Immunologic Serum

What “feature” did the His-Tag beads have to bind to protein?

• Nickel ions

• HIS

• Methyl (-CH₃)

• Polyethylene Glycol (PEG)

In HIS-tag protein purification, what had to be done to GFP before adding the GFP to the beads?

• A HIS tag was added

• The protein was precipitated with PEG

• The protein was flipped inside out

• The protein was extracted from the egg

In HIS-tag protein purification, how was a HIS-tag added to the protein?

• Genetic engineering

• The protein was mixed with PEG and centrifuged

• A high salt solution was added

• A solution free of salt was added