DNA Experiment

What plant did you use to extract DNA from?

• Onion

• Celery

• Kiwi

• Geranium

What was added to the chopped cells?

• Salt,

• Washing up liquid

• Bleach

• Pepper

What was the reason for the salt?

• To denature the enzyme

• To clump the DNA

• Break down cell membranes

• Further break down cell wall

Heat the mixture at ? for how long?

• room temperature for 3 seconds

• Room temperature for 15 seconds

• 60 degrees for 15 minutes

• 60 degrees for 15 seconds

What are the next two steps after heating it? In order.

• Let cool on the desk and blend

• Blend for 5 seconds and pour into a test tube

• Blend for 3 seconds and then Use filter paper

• Quickly cool on ice, then blend for 3 seconds.

The mixture is filtered using what?

• Coffee filter paper, because pores are larger and is quicker.

• Filter paper, because it is the only thing in the lab.

• Coffee filter paper, because the pores are really small so only allows the proper material to filter.

• Filter paper, because the pores are the perfect size.

Protease is added to the filtrate, what is protease used for?

• Separating the filtrate

• Break down the proteins to release the DNA

• It is an enzyme

What is the next step after adding protease?

• Trickling ethanol down the side of the test tube.

• Removing the DNA strands

• Trickling ice cold ethanol down the side of the test tube.

• Cleaning up after the experiment.

Why must the ethanol be ice cold

• Ethanol will evaporate at room temperature.

• DNA likes ice cold ethanol and will dissolve in it.

• Ethanol is found in an ice cold state.

• DNA is insoluble in ice cold ethanol,making it visible.