Topic 4: Enzyme kinetics

What is the role of Michaelis-Menten equation?

• To calculate the free energy

• Mathematically explain the relationship of enzyme's velocity and the substrate concentration

• Mathematically explain the saturation of enzyme in substrate

What is the meaning of these terms: E, S, ES

• Respectively for Enzyme's effectiveness, Substrate, and complex of enzyme-substrate

• Respectively for Enzyme, Substrate, and Enzyme-Substrate complex

What is the meaning of these terms: [E], [S], [ES]

• Respectively for concentration of enzyme, concentration of substrate, and concentration of enzyme-substrate complex

• Respectively for amount of enzyme, concentration of substrate, and concentration of enzyme-substrate complex

Tags

NGSS.HS-PS1-5

 $Velocity,\ V=\frac{d\left[P\right]}{dt}$  is the equation for enzyme's velocity. Which of the following information are correct? 

• Velocity is the rate of product formation over time

• Velocity is a measurement of rate

• Velocity measures the depletion (kekurangan) of the substrate

• Velocity measures the formation of the product

Tags

NGSS.HS-PS1-2

Select the correct statement(s) related to Michaelis-Menten plot (graph)

• At zero concentration of substrate, meaning [S]=0, the velocity is zero

• As the concentration of the substrate increases, the formation of product also increases

• The plot is generating using different concentration of substrate

• The plot is generating using different concentration of enzyme

Tags

NGSS.HS-PS1-2

What is Vmax?

• With a fix concentration of enzyme, the formation of product will not increase even if you add more substrate

• A maximum value for Velocity

• Related to a situation where the Michaelis-Menten plot reaches a plateau ('flat')

• Maximum substrate that is been converted to product

What is Km?

• The unit for length is cm. The unit for Km is substrate concentration

• Km is the value of [S] when the velocity is half Vmax

• Km is kilometer, how far the enzyme can move

• Km is Michaelis-Menten constant

Affinity is a word to describe how effective is the binding between the enzyme and the substrate. What is the relationship of Km with affinity?

• High Km, High affinity

• High Km, Low affinity

• With lower Km, the enzyme can reach Vmax faster