MI 1.1.5 Serial Dilution Lesson

MI 1.1.5 Serial Dilution Lesson

by LATEIRA HAYNES

​ELISAs and Serial Dilutions

​ELISAs use serial dilutions to help interpret the results of an ELISA. A Serial dilution helps us determine the concentration by using color. You compare the color of your sample to the serial dilution.

​Serial DIlution

A serial Dilution is a step-by-step dilution process for one substance into a solution.

​Solutions

​In a solution, you have your solute/sample and your solvent/diluent (what your sample is added into).

​Serial Dilution Calculations

​Serial dilution amounts are calculated as a fraction (1/x).

​

​The value of the fraction is the amount of sample (numerator) over the total volume (sample +diluent) in the tube denominator.

​Calculating Final Dilution

Multiple Choice

What is the final dilution for Tube 3?

• $$\frac{1}{20}$$  

• 1/400

• $$\frac{1}{8000}$$  

• $$\frac{1}{160000}$$  

Multiple Choice

What is the tube dilution for Tube 3?

• $$\frac{1}{20}$$  

• 1/400

• $$\frac{1}{8000}$$  

• $$\frac{1}{160000}$$  

​Calculating Concentration in Serial Dilutions

​Calculating Concentration in Serial Dilutions

Multiple Select

If 1 mL sample is put into tube #1 that has 24 ml diluent, what is the tube dilution? What is the final dilution?

• Tube 1/25

• Final 1/50

• Tube 1/50

• Final 1/25

Multiple Choice

If the concentration of the stock solution of 50 ng/ml, what is the concentration of a tube with final dilution of 1/1250?

• 0.2 ng/mL

• 50 ng/mL

• 0.04 ng/mL

• 2 ng/mL

Multiple Choice

What would be the tube dilution?

• 1/10
• 2/10
• 1/100
• 1/1000

Multiple Choice

What would be the final dilution?

• 1/10
• 1/100
• 1/1000
• 1/10000

Multiple Choice

What is the tube dilution?

• $$\frac{0.4}{9.6}$$  

• $$\frac{0.4}{10}$$  

• $$\frac{1}{25}$$  

• $$\frac{4}{100}$$  

Multiple Choice

What is the tube dilution?

• $$\frac{50}{100}$$  

• $$\frac{1}{20}$$  

• $$\frac{50}{150}$$  

• $$\frac{1}{3}$$  

Poll

How do you feel about serial dilutions?

• I completely understand.

• I think I understand but, I need more practice.

• Can you go over tube dilution vs. final dilution again?

• I have no idea how to do this.