Introduction to the Light Microscope

Front

What is a light microscope?

Back

A light microscope is an optical instrument that uses visible light and a system of lenses to magnify small objects, such as cells and microorganisms.

Front

What is the typical magnification of an ocular lens?

Back

10X

Front

What is the purpose of the objective lens in a light microscope?

Back

The objective lens is responsible for gathering light from the specimen and magnifying the image.

Front

What is the first step in learning about a microscope?

Back

Go to http://www.ncbionetwork.org/iet/microscope/.

Front

What is the main purpose of using a light microscope in biological studies?

Back

To magnify small organisms and cells that cannot be seen with the naked eye.

Front

Why are stains (dyes) used when preparing slides for viewing under a microscope?

Back

To color the cells so they are more easily viewed.

Front

What is the function of the stage on a light microscope?

Back

The stage is the platform where the slide is placed for observation.

Front

What is the role of the diaphragm in a light microscope?

Back

The diaphragm controls the amount of light that reaches the specimen.

Front

What is the purpose of the coarse focus knob?

Back

The coarse focus knob is used to make large adjustments to the focus of the microscope.

Front

What is the purpose of the fine focus knob?

Back

The fine focus knob is used for making small adjustments to the focus for clearer images.