MCB lab final

Front

What is the positive control for the Crystal Violet assay?

Back

Peroxide is used as the positive control in the Crystal Violet assay.

Front

What are the two main stages of the cell cycle?

Back

The cell cycle is divided into interphase and mitosis.

Front

What type of cells does Propidium Iodide stain and what is its permeability characteristic?

Back

Propidium Iodide stains dead cells and is non-permeable, meaning it can only enter cells with compromised membranes.

Front

What is the nature of the Crystal Violet assay and the InCell Analyzer in determining EC 50?

Back

The Crystal Violet assay is qualitative, while the InCell Analyzer is quantitative.

Front

What does fluorescence intensity in flow cytometry correlate with?

Back

Fluorescence intensity is directly proportional to the amount of DNA.

Front

Define interphase in the context of the cell cycle.

Back

Interphase is the stage of the cell cycle where the cell prepares for mitosis, including DNA replication.

Front

What is the role of Crystal Violet in cell assays?

Back

Crystal Violet is a dye used to stain cells, allowing for the assessment of cell viability.

Front

Explain the significance of using a positive control in experiments.

Back

A positive control is used to validate that the experimental setup is capable of producing a positive result.

Front

What is the difference between qualitative and quantitative assays?

Back

Qualitative assays provide descriptive data, while quantitative assays provide numerical data.

Front

What does it mean for a molecule to be non-permeable?

Back

A non-permeable molecule cannot pass through the cell membrane without assistance.