PCR Flashcard

Flashcard
•
Chemistry, Biology
•
9th - 12th Grade
•
Hard
Wayground Content
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15 questions
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1.
FLASHCARD QUESTION
Front
What is PCR?
Back
PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA sequences, making millions of copies of a particular segment of DNA.
2.
FLASHCARD QUESTION
Front
What happens in the Denature step of PCR?
Back
The double-stranded DNA is separated into two single strands of DNA.
3.
FLASHCARD QUESTION
Front
At what temperature does the Denature step of PCR occur?
Back
94-96° C.
4.
FLASHCARD QUESTION
Front
What is the purpose of the Denature step in PCR?
Back
To separate the double-stranded DNA into single strands, allowing access for primers.
5.
FLASHCARD QUESTION
Front
What happens in the Annealing step of PCR?
Back
Primers bind to the single-stranded DNA templates.
6.
FLASHCARD QUESTION
Front
At what temperature does the Annealing step of PCR occur?
Back
50-65° C.
7.
FLASHCARD QUESTION
Front
What is the purpose of the Annealing step in PCR?
Back
To allow primers to attach to the complementary sequences on the single-stranded DNA.
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