High Performance Liquid Chromatography (HPLC)

Front

What is High Performance Liquid Chromatography (HPLC)?

Back

A form of column chromatography that pumps a sample mixture in a solvent at high pressure through a column with chromatographic packing material.

Front

Why is HPLC widely used?

Back

It provides sensitive, accurate quantitative analysis and can separate non-volatile and thermally unstable compounds.

Front

What are the two phases required in HPLC?

Back

Mobile Phase and Stationary Phase.

Front

What is the role of the Mobile Phase in HPLC?

Back

It drives the mixture through the stationary phase with the aid of a pump.

Front

What is the Stationary Phase in HPLC?

Back

It is a column packed with silica gel, which is essential for the separation of analytes.

Front

List the components of HPLC.

Back

A - Mobile Phase Reservoir, B - Pump, C - Sample Injector, D - Column (Stationary Phase), E - Detector, F - Data Analyser, G - Waste Container.

Front

Describe the HPLC process briefly.

Back

The mobile phase is pumped to the column, the sample is injected into the mobile phase stream, and components are separated based on their affinity to the mobile or stationary phase.

Front

What happens to the component with the greatest affinity for the mobile phase in HPLC?

Back

It reaches the detector first.

Front

What happens to the component with the greatest affinity for the stationary phase in HPLC?

Back

It is the last component to reach the detector.