Gel Electrophoresis Practice

Front

What is a Point Mutation?

Back

A type of mutation where one nucleotide base is changed, affecting only one amino acid in the protein sequence.

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What is a Substitution Mutation?

Back

A mutation where one base pair is replaced by another, which can lead to changes in the amino acid sequence.

Front

What is a Silent Mutation?

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A substitution mutation that does not change the amino acid sequence of a protein.

Front

What is the function of Mitochondria?

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Mitochondria are organelles that produce ATP, the energy currency of the cell.

Front

What is Gel Electrophoresis?

Back

A laboratory method used to separate mixtures of DNA, RNA, or proteins according to their size.

Front

What is the role of DNA in Gel Electrophoresis?

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DNA fragments are separated based on size, allowing for analysis of genetic material.

Front

What is the significance of the size of DNA fragments in Gel Electrophoresis?

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Smaller DNA fragments move faster through the gel, allowing for size-based separation.

Front

What is the purpose of a DNA ladder in Gel Electrophoresis?

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A DNA ladder is a set of known DNA fragments used as a reference to determine the size of unknown DNA samples.

Front

What is the role of agarose gel in Gel Electrophoresis?

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Agarose gel acts as a medium through which DNA fragments can migrate during electrophoresis.

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What is the principle of electrophoresis?

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Electrophoresis is based on the movement of charged particles in an electric field.