DNA Replication & PCR (2.7, 7.1)

Front

What is the semi-conservative model of DNA replication?

Back

The semi-conservative model of DNA replication states that each new DNA molecule consists of one original strand and one newly synthesized strand.

Front

Who were the scientists that proved DNA replication is semi-conservative?

Back

Meselson and Stahl.

Front

What is the difference between the leading strand and the lagging strand during DNA replication?

Back

The leading strand is replicated continuously, while the lagging strand is replicated discontinuously in short segments called Okazaki fragments.

Front

What are the end products of DNA replication?

Back

Two DNA molecules, each containing one strand of the original DNA molecule.

Front

What is the role of primers in PCR?

Back

Primers are short sequences of nucleotides that bind to the template DNA and provide a starting point for DNA synthesis.

Front

What happens during the annealing step of PCR?

Back

During the annealing step, primers bind to the template DNA.

Front

What is the function of Taq DNA polymerase in PCR?

Back

Taq DNA polymerase is an enzyme that synthesizes new DNA strands by adding nucleotides to the primers.

Front

What is the significance of using different primers in PCR tests?

Back

Different primers are used to target specific DNA sequences, allowing for the detection of different alleles or genes.

Front

What are nucleotides and their role in DNA replication?

Back

Nucleotides are the building blocks of DNA, consisting of a sugar, a phosphate group, and a nitrogenous base. They are added to the growing DNA strand during replication.

Front

What is the purpose of denaturation in PCR?

Back

Denaturation is the first step in PCR where the double-stranded DNA is heated to separate it into two single strands.