MI U4 GFP Lab Mod 4

MI U4 GFP Lab Mod 4

Assessment

Flashcard

Biology

9th - 12th Grade

Hard

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15 questions

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1.

FLASHCARD QUESTION

Front

What is a plastic cassette in the context of gel electrophoresis?

Back

A plastic cassette is a device that holds the gels used in gel electrophoresis, structured like a sandwich of plastic-gel-plastic.

2.

FLASHCARD QUESTION

Front

Does denatured GFP glow under UV light?

Back

No, denatured GFP does not glow under UV light.

3.

FLASHCARD QUESTION

Front

Why must gels be handled with care?

Back

Gels are very delicate and can be easily damaged, requiring extreme care during and after removal from the cassette.

4.

FLASHCARD QUESTION

Front

What is one method to denature proteins?

Back

Boiling them is a common method to denature proteins.

5.

FLASHCARD QUESTION

Front

Is staining the gels a one-step process?

Back

No, staining the gels typically involves multiple steps, not just covering with stain and draining excess.

6.

FLASHCARD QUESTION

Front

What is gel electrophoresis used for?

Back

Gel electrophoresis is used to separate and analyze macromolecules like DNA, RNA, and proteins based on their size and charge.

7.

FLASHCARD QUESTION

Front

What happens to proteins when they are denatured?

Back

Denatured proteins lose their natural structure and function, which can affect their behavior in experiments.

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