What is a plastic cassette in the context of gel electrophoresis?
MI U4 GFP Lab Mod 4

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Biology
•
9th - 12th Grade
•
Hard
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1.
FLASHCARD QUESTION
Front
Back
A plastic cassette is a device that holds the gels used in gel electrophoresis, structured like a sandwich of plastic-gel-plastic.
2.
FLASHCARD QUESTION
Front
Does denatured GFP glow under UV light?
Back
No, denatured GFP does not glow under UV light.
3.
FLASHCARD QUESTION
Front
Why must gels be handled with care?
Back
Gels are very delicate and can be easily damaged, requiring extreme care during and after removal from the cassette.
4.
FLASHCARD QUESTION
Front
What is one method to denature proteins?
Back
Boiling them is a common method to denature proteins.
5.
FLASHCARD QUESTION
Front
Is staining the gels a one-step process?
Back
No, staining the gels typically involves multiple steps, not just covering with stain and draining excess.
6.
FLASHCARD QUESTION
Front
What is gel electrophoresis used for?
Back
Gel electrophoresis is used to separate and analyze macromolecules like DNA, RNA, and proteins based on their size and charge.
7.
FLASHCARD QUESTION
Front
What happens to proteins when they are denatured?
Back
Denatured proteins lose their natural structure and function, which can affect their behavior in experiments.
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