Sanger Method Flashcard

Front

What role do fluorescent dideoxynucleotides play in the Sanger sequencing method?

Back

They terminate DNA synthesis and allow for detection.

Front

What is the significance of the chain termination method in Sanger sequencing?

Back

It enables the determination of the DNA sequence by terminating synthesis at specific nucleotides.

Front

What is the role of the DNA template in the Polymerase Chain Reaction (PCR) for the Sanger sequencing method?

Back

To serve as the starting material for DNA synthesis.

Front

Which component is not required for the Polymerase Chain Reaction (PCR) in the Sanger method?

Back

RNA polymerase.

Front

What is the purpose of gel electrophoresis in the Sanger sequencing method?

Back

To separate DNA fragments based on size.

Front

Define Sanger sequencing.

Back

A method for determining the nucleotide sequence of DNA using chain-terminating dideoxynucleotides.

Front

What are dideoxynucleotides?

Back

Modified nucleotides that terminate DNA strand elongation during sequencing.

Front

Explain the role of primers in Sanger sequencing.

Back

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis.

Front

What is the function of DNA polymerase in Sanger sequencing?

Back

DNA polymerase synthesizes new DNA strands by adding nucleotides complementary to the template strand.

Front

How does chain termination allow for DNA sequencing?

Back

By incorporating dideoxynucleotides, which prevent further elongation, resulting in fragments of varying lengths that can be analyzed.