Tools for Genetic Engineering

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What are restriction enzymes?

Back

Enzymes that cut DNA at specific sequences, producing either sticky or blunt ends.

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What are sticky ends in DNA?

Back

Single-stranded ends of DNA that can easily bond with complementary DNA strands.

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What are blunt ends in DNA?

Back

Straight cut ends of DNA that do not have overhanging nucleotides.

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What is a plasmid?

Back

A circular piece of DNA found in bacteria, often used as a vector in genetic engineering.

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What is the role of genes in DNA?

Back

Genes contain the instructions for making proteins.

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What is reverse transcription?

Back

The process of producing DNA from an mRNA template.

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What is a vector in genetic engineering?

Back

Structures that deliver genes into required cells.

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What is the function of DNA ligase?

Back

An enzyme that joins DNA fragments together by forming phosphodiester bonds.

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What is PCR (Polymerase Chain Reaction)?

Back

A technique used to amplify a specific DNA segment, making millions of copies.

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What is the purpose of gel electrophoresis?

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A method used to separate DNA fragments based on their size.