Quick 10Q: BioTechnology

Flashcard
•
Biology
•
9th - 12th Grade
•
Hard
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15 questions
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1.
FLASHCARD QUESTION
Front
What is the result of 1 DNA fragment after 5 cycles of PCR?
Back
32 fragments.
2.
FLASHCARD QUESTION
Front
What is the minimum number of restriction sites on a plasmid with 3 fragments on a gel?
Back
3 restriction sites.
3.
FLASHCARD QUESTION
Front
Why must primers be added to the thermocycler during PCR?
Back
Polymerase requires an open 3' end.
4.
FLASHCARD QUESTION
Front
Why are some bands darker in gel electrophoresis?
Back
There are a large amount of fragments the same size.
5.
FLASHCARD QUESTION
Front
What process allows for the amplification of a DNA fragment abiotically?
Back
Polymerase Chain Reaction (PCR).
6.
FLASHCARD QUESTION
Front
Define Polymerase Chain Reaction (PCR).
Back
A technique used to amplify DNA segments.
7.
FLASHCARD QUESTION
Front
What role do primers play in PCR?
Back
Primers provide a starting point for DNA synthesis.
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