DNA Fingerprinting Test

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What is DNA Fingerprinting?

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A technique used to identify individuals based on their unique DNA profiles.

Front

What role do restriction enzymes play in DNA analysis?

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They cut DNA into fragments of different lengths, which is essential for DNA fingerprinting.

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What is the purpose of PCR in DNA analysis?

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PCR (Polymerase Chain Reaction) is used to make millions of copies of a specific DNA segment.

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What does gel electrophoresis do?

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It separates DNA fragments based on their size, allowing for analysis of the DNA.

Front

What indicates contamination in a DNA fingerprinting test?

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Bands appearing in the negative control lane indicate contamination and invalid results.

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What is a negative control in DNA testing?

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A sample that should not show any DNA bands, used to check for contamination.

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How does DNA size affect its movement in gel electrophoresis?

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Smaller DNA fragments travel further through the gel than larger fragments.

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What is the significance of the positive control in DNA testing?

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It confirms that the test is working properly by showing expected results.

Front

What does it mean if a DNA sample shows no bands in gel electrophoresis?

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It may indicate that the sample did not contain DNA or that the DNA was degraded.

Front

What is the function of DNA polymerase in PCR?

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DNA polymerase synthesizes new DNA strands by adding nucleotides to the template strand.