PCR Flashcard

Front

What is the purpose of PCR?

Back

To produce millions of copies of a specific region of DNA.

Front

What are the base-pairing rules in DNA?

Back

A-T, G-C.

Front

At what temperature does the Denature step of PCR occur?

Back

94-96° C.

Front

What does PCR stand for?

Back

Polymerase Chain Reaction.

Front

How many cycles of PCR are normally used?

Back

20-35.

Front

What is the role of DNA polymerase in PCR?

Back

To synthesize new DNA strands by adding nucleotides.

Front

What are the three main steps of PCR?

Back

Denaturation, Annealing, Extension.

Front

What is denaturation in PCR?

Back

The process of heating the DNA to separate its strands.

Front

What is annealing in PCR?

Back

The process of cooling the reaction to allow primers to bind to the template DNA.

Front

What is extension in PCR?

Back

The process where DNA polymerase adds nucleotides to the growing DNA strand.