Gram Staining Activity Flashcard

Front

What is Gram Staining?

Back

A laboratory technique used to differentiate bacterial species into two groups based on the characteristics of their cell walls.

Front

What are the two main types of bacteria identified by Gram Staining?

Back

Gram-positive and Gram-negative.

Front

What color do Gram-positive bacteria stain?

Back

Purple.

Front

What color do Gram-negative bacteria stain?

Back

Pink.

Front

What is the purpose of the primary stain in Gram Staining?

Back

To initially stain all the cells, allowing for differentiation later.

Front

What role does iodine play in Gram Staining?

Back

Iodine acts as a mordant, helping the primary stain adhere to the bacterial cell wall.

Front

What is the purpose of the decolorization step in Gram Staining?

Back

To wash out the primary stain from Gram-negative bacteria, allowing for differentiation.

Front

What is the counter stain used in Gram Staining?

Back

Safranin.

Front

What is peptidoglycan?

Back

A polymer that makes up the cell wall of bacteria, providing structural support.

Front

How does the cell wall structure differ between Gram-positive and Gram-negative bacteria?

Back

Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer and an outer membrane.