MBT FL24 Transformation

Front

What is transformation in genetic engineering?

Back

The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

Front

Why is calcium chloride used in the transformation process?

Back

The cations from CaCl2 allow for the negative plasmid to get close to the negative plasma membrane.

Front

What is the correct sequence for the process of genetic transformation?

Back

1. Bacterial cells transferred to cold CaCl2 2. Plasmid with foreign DNA added to bacteria 3. Cells heat shocked at 42°C 4. Cells spread on agar petri dish 5. Bacteria given time to grow on petri dish.

Front

How can a human gene be expressed in a bacterial cell?

Back

The DNA structure and code is the same in all living organisms; therefore, bacteria contain the same cell machinery for performing transcription and translation and can read the same DNA as human cells.

Front

What process increases the competency of cells?

Back

Calcium chloride and heat shock.

Front

What is a plasmid?

Back

A plasmid is a small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently within a bacterial cell.

Front

What role does heat shock play in transformation?

Back

Heat shock creates a temperature difference that helps to facilitate the uptake of plasmid DNA by bacterial cells.

Front

What is the purpose of using agar petri dishes in transformation?

Back

Agar petri dishes provide a solid medium for bacteria to grow and allow for the selection of transformed cells.

Front

What is competency in the context of bacterial cells?

Back

Competency refers to the ability of bacterial cells to take up foreign DNA from their environment.

Front

What is the significance of using ice-cold calcium chloride?

Back

Ice-cold calcium chloride helps to stabilize the bacterial cell membrane and prepares the cells for heat shock.