Biotechnology HW 8.6

Biotechnology HW 8.6

Assessment

Flashcard

Science

12th Grade

Hard

Created by

Wayground Content

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15 questions

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1.

FLASHCARD QUESTION

Front

What is the final step in the miniprep procedure before storing the plasmids?

Back

Resuspending the plasmid pellet in TE buffer.

2.

FLASHCARD QUESTION

Front

What is the purpose of using restriction enzymes in the extracted plasmid sample?

Back

To confirm plasmid size and sequence.

3.

FLASHCARD QUESTION

Front

What is the purpose of adding isopropanol during the miniprep procedure?

Back

To precipitate nucleic acids.

4.

FLASHCARD QUESTION

Front

What is one advantage of using commercial kits for plasmid preparation?

Back

They provide pre-made buffers and solutions.

5.

FLASHCARD QUESTION

Front

What is the name of the procedure in which plasmids are extracted from cells?

Back

Plasmid prep.

6.

FLASHCARD QUESTION

Front

What is a plasmid?

Back

A small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently.

7.

FLASHCARD QUESTION

Front

What is TE buffer and its role in plasmid storage?

Back

TE buffer is a solution of Tris-EDTA that helps to stabilize DNA during storage.

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