Biotechnology HW 8.6
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•
Science
•
12th Grade
•
Hard
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1.
FLASHCARD QUESTION
Front
What is the final step in the miniprep procedure before storing the plasmids?
Back
Resuspending the plasmid pellet in TE buffer.
2.
FLASHCARD QUESTION
Front
What is the purpose of using restriction enzymes in the extracted plasmid sample?
Back
To confirm plasmid size and sequence.
3.
FLASHCARD QUESTION
Front
What is the purpose of adding isopropanol during the miniprep procedure?
Back
To precipitate nucleic acids.
4.
FLASHCARD QUESTION
Front
What is one advantage of using commercial kits for plasmid preparation?
Back
They provide pre-made buffers and solutions.
5.
FLASHCARD QUESTION
Front
What is the name of the procedure in which plasmids are extracted from cells?
Back
Plasmid prep.
6.
FLASHCARD QUESTION
Front
What is a plasmid?
Back
A small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently.
7.
FLASHCARD QUESTION
Front
What is TE buffer and its role in plasmid storage?
Back
TE buffer is a solution of Tris-EDTA that helps to stabilize DNA during storage.
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