Biotechnology

Front

During PCR, DNA sample is cooled to 54°C, allowing primers to attach to opposite ends of the target sequence. That step in the PCR is called:

Back

Annealing

Front

Name the enzyme that joins the sticky ends, fixing the gene into the plasmid.

Back

DNA Ligase

Front

Clone

Back

A group of genetically-identical organisms derived from a single original parent cell

Front

Gel electrophoresis is a laboratory technique used to

Back

Separate and isolate proteins or DNA fragments based on size

Front

The transfer of genes between species is called gene modification, and the new organism created is called a

Back

Transgenic

Front

Can certain animal species reproduce asexually and can humans create genetic clones?

Back

True

Front

This image is a photograph of DNA fragments that have been separated. Identify which strand of DNA is the shortest. Options: A, B, C, D

Back

B

Front

To compare a specific region of DNA from a 100-year-old preserved Tasmanian wolf with living marsupials, which method increases the amount of wolf DNA for testing? Options: DNA profiling, Karyotyping, PCR, Gel electrophoresis.

Back

PCR

Front

Which is NOT a standard part of PCR?

• Use of a heat-resistant polymerase
• Breaking of the hydrogen bonds that connect two complementary DNA strands
• Supply of large numbers of A, C, G and T nucleoside triphosphates
• Breaking of covalent bonds within the backbone of DNA strands

Back

Breaking of covalent bonds within the backbone of DNA strands

Front

Why are plasmids useful in genetic modification of bacteria?

Back

They are easily transferred between bacterial cells and are small enough to manipulate easily.